Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotang Inc TRIS-HCL Buffer 1.0M, pH 8.4 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 8.4 (0.2 micron filtered), 500 ml.
Biotang Inc TRIS-HCL Buffer 1.0M, pH 8.5 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 8.5 (0.2 micron filtered), 500 ml.
Teknova 10X TBE DNA Sequencing Grade Solution. 1000mL, Non-Sterile 2 pack.
890mM Tris,890mM Boric Acid, 20mM Ethylenediaminetetraacetic acid (EDTA)
Hampton Research Tris Buffer
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL) Please note items from this supplier are non-returnable.
Thermo Scientific Production Chemicals and Services 10MM TRIS-HCL 1MM EDTA
NC3588246 10MM TRIS-HCL 1MM EDTA
Thermo Scientific Production Chemicals and Services 10MM TRIS-HCL 1MM EDTA PH100L
NC3588256 10MM TRIS-HCL 1MM EDTA PH100L
Alkali Scientific TRIS Glycine SDS Buffer, 10X Powder, 184 Grams of Powder, Makes 1 Liter of Solution
TRIS Glycine SDS Buffer (10X Powder) is a commonly used buffer solution in electrophoresis and protein separation techniques. This 184g powder is designed to make 1 liter of 10X concentrated solution, ideal for preparing protein gels for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). The buffer helps maintain a consistent pH and conductivity during protein separation, ensuring reproducible results. It is widely used in laboratories for protein analysis, molecular biology applications, and biochemistry experiments. TRIS Glycine SDS Buffer is essential for the proper functioning of electrophoresis systems, helping researchers isolate proteins based on size and charge in a reliable and efficient manner.
Kd Medical Inc TRIS BUFFER, 2M, PH 7.5, 1L
Buffer used in a wide range of Molecular Biology applications. 2 Molar solution pH 7.5. One 1 Liter bottle.