Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc HEPES Buffered Saline (5X, pH 7.4) - 500ml
HEPES Buffered Saline (5X pH 7 4) - 500ml (For Research Use and Further Manufacturing Only)
Bioworld EGTA 0.5M, PH 8.0,250ML
EGTA 0.5M, pH 8.0, DNase and RNase Free,250ml.EGTA Buffer is of a 0.5M concentration and pH of 8.0 that is DNase and RNase free. EGTA Buffer is a metal chelating agent specific for calcium ions. EGTA Buffer is commonly used in tissue culture media and biological buffers.
Teknova 1M Tris-HCl, pH 7.5. 500ml Sterile. 4 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova SM Buffer. 1000mL, Sterile 2 pack.
100mM Sodium Chloride, 8mM Magnesium Sulfate,0.01% Gelatin, 50mM Tris-HCl
Teknova Tris Glycerol Buffer pH 8.0. 1000mL, Endotoxin Tested, Sterile 2 pack.
20mM Tris,25mM Sodium Chloride,2.5% Glycerol
Teknova 2M Tris-HCl, pH 7.5 Solution. 1000mL, Sterile. 2 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova 0.45% Saline Solution, Sterile. 500mL 4 pack.
Sodium chloride is an essential ingredient in the lab. Not only does it help regulate the pH of solutions, but it also prevents growth of bacteria and other contaminants. In addition, salt is a powerful desiccant, meaning that it can help to dry out wet samples. As a result, salt is often used in a wide variety of laboratory procedures.
![Alkali Scientific HEPPS [N (2 Hydroxyethyl)piperazine N' (3 propane sulfonic acid)] [EPPS], 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660189.jpg-250.jpg)
Alkali Scientific HEPPS [N (2 Hydroxyethyl)piperazine N' (3 propane sulfonic acid)] [EPPS], 1 Kg
HEPPS (N-(2-Hydroxyethyl)piperazine-N'-(3-propane sulfonic acid)) is a biochemical reagent supplied in a 1 kg bottle. This high-quality buffering agent is commonly used in laboratory and industrial applications for maintaining stable pH conditions, particularly for enzyme assays, protein studies, and cell culture applications. HEPPS is particularly useful in biological research, as it maintains pH stability under a wide range of experimental conditions. Its use in protein purification and electrophoresis helps researchers obtain precise and consistent results. This 1kg bottle is ideal for high-throughput laboratories or research settings requiring large quantities of buffering solution.
![Alkali Scientific PIPES [Piperazine N,N' bis (2 ethanesulfonic acid)], 10 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659951.jpg-250.jpg)
Alkali Scientific PIPES [Piperazine N,N' bis (2 ethanesulfonic acid)], 10 G
PIPES (Piperazine N,N' bis (2 ethanesulfonic acid)) is a biochemical reagent commonly used in buffer solutions for various laboratory applications. This 10g formulation provides a stable pH environment and is ideal for maintaining consistent acidity in biochemical and microbiological experiments. PIPES buffers are widely used in protein chemistry, electrophoresis, and cell culture studies due to their high buffering capacity at a physiological pH. This reagent is commonly used in applications requiring a neutral pH (around 7.0) to stabilize the environment and prevent pH fluctuations that could impact experimental results.
![Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], 50 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659952.jpg-250.jpg)
Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], 50 G
PIPES (Piperazine N,N' bis(2 ethanesulfonic acid)) is a highly stable biochemical reagent used to prepare buffer solutions that maintain a consistent pH. This 50g quantity is ideal for laboratories requiring precise pH control in applications such as protein chemistry, enzyme kinetics, and electrophoresis. PIPES buffers are used in biological assays where maintaining a neutral pH (around 7.0) is critical for enzyme activity and the stability of proteins. This versatile buffer is commonly employed in cellular biology, microbiology, and various biochemical processes that need accurate and reliable pH regulation.
![Alkali Scientific MOPS [3 (N morpholino) propanesulfonic acid], 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660062.jpg-250.jpg)
Alkali Scientific MOPS [3 (N morpholino) propanesulfonic acid], 500 G
MOPS (3-(N-morpholino)propane-1-sulfonic acid) is a versatile biochemical reagent used to maintain pH stability in laboratory experiments. This 500g package is suitable for medium to high-volume buffer preparations required in molecular biology, protein analysis, and biochemical assays. MOPS is commonly used in electrophoresis, chromatography, and enzyme assays where maintaining a consistent pH in the range of 6.5 to 7.9 is essential. It is particularly useful in applications that involve the stabilization of proteins, enzymes, and nucleic acids, making it a critical reagent in molecular research, cell biology, and biochemistry laboratories.
Sigma Aldrich Fine Chemicals Biosciences BICINE Redi DriTM 99 free 500G
BICINE Redi DriTM 99 free 500G
![Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660279.jpg-250.jpg)
Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 100 G
HEPES, Free Acid (N-(2-Hydroxyethyl) Piperazine-N'-(2-Ethanesulfonic Acid)) is a biochemical reagent commonly used as a buffering agent in biological and chemical research. The free acid form of HEPES is highly effective at maintaining pH stability in biological assays, particularly in environments where pH needs to be tightly controlled. This 100g package size is ideal for smaller laboratories or experiments that require high-purity HEPES for preparing buffer solutions. HEPES is widely used in molecular biology, cell culture, and microbiology for its low interference in biological reactions, ensuring accurate and reproducible results in a variety of applications.
